CD163 and neoplasm: In brief, raw mIF images (Figure 1B) were segmented to identify single cells (Figure 1C), and individual cells were phenotyped into the following 10 phenotypes (Figures 1D and 1E): Tumor cells (PAX5+ cells) with (1) and without (2) PD-L1 expression, T cells (CD3+ cells) and their subtypes classified by expression of CD8 and PD-1 (3–6), macrophages (CD163+ cells) with (7) and without (8) PD-L1 expression, and other cell types with (9) and without (10) PD-L1 expression (Figure 1B).