FTO and acute myeloid leukemia: To better elucidate the mechanisms by which FTO intron 8 elements regulate IRX3 activation, we analyzed RNAseq data from the primary AML samples used for our 4Cseq and ChIPseq experiments and noted transcripts arising from putative enhancer E2 running through to the last exon of FTO. These were only detected in IRX3high samples (Figure 3A).