To identify regulatory elements that might control the expression of IRX3 within the TAD, we compared mean H3K27Ac chromatin immunoprecipitation (ChIP) signal in 26 IRX3high versus 28 IRX3low primary AML samples from a published dataset comprising samples from a range of molecular subtypes14 (Figure 1A; Table S1). This evidence concerns the gene IRX3 and acute myeloid leukemia.