These show that IRX3 lies within a TAD which also contains two other members of the Iroquois homeobox family (IRX5 and IRX6), the protein-coding genes FTO and RPGRIP1L, and the long non-coding RNA CRNDE. To provide additional confirmation in an independent AML cell line, we performed 4Cseq in Fujioka cells which express IRX3 at a high level (Figure S1C) using a viewpoint within the IRX3 promoter and found that the pattern of interactions was restricted to the predicted IRX3 TAD (Figure S1C). Here, FTO is linked to acute myeloid leukemia.