The results obtained in this study appear toowe in part to the properties of MCF7 cells, with high-EGFR MDA-MB-231 cellsnot forming spheroids without the addition of growth factors and matrices.Nevertheless, developing such a cell model with abnormal N-cadherin activationis essential for identifying potential molecular targets of tumor progression.Moreover, MCF7-EGFR spheroids are considered to be a model for testing thetherapeutic effects of the combination of EGFR and N-cadherin inhibitors. This evidence concerns the gene EGFR and neoplasm.