In GBM patient samples, 8 elevated biomarkers, such as BMP1, CTSB, LOX, LOXL1, PLOD1, MMP9, SERPINE1, and SERPING1, were linked to poor prognosis in patients, and only BMP1, HIF1A, and TNFRSF1B, have been identified as substrates involved in the ubiquitination process corresponding E3 ligases. The gene discussed is CTSB; the disease is glioblastoma.