Intriguingly, an increased portion of upregulated SIRT3‐targeted proteins also showed in icSIRT1−/− group as compared to the SIRT1f/f group during acute ischemia and I/R stress (Figure 4c), indicating that cardiac SIRT1 deficiency in aging alters SIRT3‐associated metabolic proteins in response to acute ischemia and I/R stress. Here, SIRT1 is linked to ischemia.