To clarify the role of HMGB1 in cytosolic translocation during chemotherapy, we analyzed the responses of colorectal cancer cells to naphplatin by adding ethyl pyruvate (EP), a pharmacological inhibitor of HMGB1 cytoplasmic translocation (Fig. S52c, g, 5g, h, S53, and S54), and observed an enhanced cell proliferation and migration in CT-26 (Fig. S53a, b, and S54a, c, d) and HCT-116 (Fig. S53c, d and S54b, e, f) cells. This evidence concerns the gene HMGB1 and colorectal cancer.