Our proteomic and transcriptomic analysis results uncovered that LINC02159 bound to Aly/REF export factor (ALYREF) and enhanced the 5-methylcytosine (m5C) modification of YAP1 mRNA by ALYREF, which upregulated YAP1 expression and activated Hippo and β-catenin pathways in NSCLC cells. This evidence concerns the gene YAP1 and non-small cell lung carcinoma.