To address the translational relevance, we performed in vitro cytotoxicity experiments using the M202 human melanoma line (human leukocyte antigen (HLA)-A*0201), which expresses the MART-1 tumor-associated antigen, or an MHC-I-negative B2M-KO version of the same cells (M202-β2mKO). The gene discussed is B2M; the disease is melanoma.