TP53 and breast cancer: Besides using nuclear extract, we also purified from bacteria the glutathione S-transferase (GST)-p53 recombinant fusion proteins, including WT p53 and various mutants such as R175H (equivalent to R172H in GEM mice), C238Y (LuCaP 23.1 patient-derived xenograft (PDX)), R248W (VCaP cell line), R273H (MDA-MB-468 breast cancer cell line) and Q331R (22Rv1 PCa cells), a residue outside of DBD.