Moreover, to further explore the effects of IL13 in the supernatant of TNF+Tregs on GC cells, we treated cells with a neutralizing antibody against IL13 and showed that neutralizing IL13 significantly attenuated the effects of the supernatant from TNF+ Tregs on the proliferation, migration, sphere formation and colony formation of gastric cancer cells (Figures 6A–G). This evidence concerns the gene IL13 and gastric cancer.