We further investigated whether the antiglioma mechanism of momordicine I is related to cell cycle modification and whether Disks Large-Associated Protein 5 (DLGAP5) is the gene encoding the potential downstream target of momordicine I. Considering the results of the current study, we believe that momordicine I treatment has the potential to be a novel therapeutic strategy for glioma. Here, DLGAP5 is linked to central nervous system cancer.