Based on the IMC protocols for liver cancer and breast cancer,47,48 we established a 35-marker panel for heart tissue, including markers for endothelial cells, immune cells, and fibroblasts, the proliferation marker Ki-67, and the apoptotic marker cleaved caspase-3 (Supplementary material online, Figure S8 and Table S5). The gene discussed is MKI67; the disease is breast carcinoma.