According to KEGG pathway analysis, we found that signaling pathways regulating pluripotency, PI3K/Akt signaling mechanism, Mucin type O-Glycan biosynthesis, ECM-receptor interaction, proteoglycans in cancer, and TGF-β signaling pathway were targeted in Bag-1KO MCF-7 cells (Figure 2A). Here, TGFB1 is linked to cancer.