In this study, we aimed to investigate a) the histopathological changes including the formation of edema, pyknotic nuclei, and hemorrhage in the medulla oblongata after CIS administration, b) the alterations in the expressions of Aqp-1 and Aqp-4 genes, c) the effects of MEL as a protective co-treatment by reducing edema and regulating the gene expressions. Here, AQP1 is linked to in situ carcinoma.