The direct effects of PGE2 on sepsis-related ALI in CLP mice were also determined, and the results showed a reduction in NET formation (Additional file 1: Fig. S10a-c), trafficking of neutrophils from bone marrow to the circulation (Additional file 1: Fig. S10d) and lung tissues (Additional file 1: Fig. S10e), and decreased expression of CXCR2 and ROS (Additional file 1: Fig. S10f-g) in peripheral blood neutrophils. The gene discussed is CXCR2; the disease is Sepsis.