However, the biological significance of UCP1 and potential regulatory mechanisms in the development of CKD remain unclear.<h4>Methods</h4>Unilateral ureteral obstruction (UUO) model was used to construct the animal model of renal fibrosis, and TGF-β1 stimulation of HK2 cells was used to construct the vitro model of renal fibrosis. Here, TGFB1 is linked to chronic kidney disease.