Unsupervised clustering showed the presence of different cell populations including tumour cells (expressing EpCAM, PGR and TFF3—all markers of ECs21), immune cells (marked by CD45 (PTPRC) expression), cancer-associated fibroblasts (CAFs, marked by αSMA (ACTA2)) and endothelial cells (PECAM1 positive) (Fig. 5b and Extended Data Fig. 4a,b). This evidence concerns the gene PTPRC and neoplasm.