Through transcriptome analysis, several signaling pathways that may be affected by PSE have been identified, such as apoptosis, PI3K-AKT, p53 signaling pathway, cell cycle, etc. To verify the expression of some major differentially expressed genes in melanoma cells, and to verify the accuracy of transcriptome analysis as well, we selected nine genes for RT-qPCR verification. The gene discussed is TP53; the disease is melanoma.