The hAbeta/APOE4/Trem2*R47H mice used to assess lipofuscin accumulation did not exhibit any visible MX04-labeled plaques by 18 months of age, so we instead implanted microelectrodes in the cortex of APP/PS1 mice, which is a transgenic AD mouse model that naturally develops large, visibly identifiable plaques by 6 months of age (supplementary figure 2). This evidence concerns the gene TREM2 and Alzheimer disease.