Finally, to further verify the molecular mechanism of EHMT2 affecting ischemic stroke by regulating HMOX1 expression, we co-transfected BV-2 cells in Control and OGD/R groups with sh-NC, sh-EHMT2, OE-NC or OE-HMOX1 plasmids (OGD/R + sh-NC + OE-NC, OGD/R + sh-EHMT2 + OE-NC, OGD/R + sh-NC + OE-HMOX1, and OGD/R + sh-EHMT2 + OE-HMOX1 groups). The gene discussed is HMOX1; the disease is ischemic stroke.