In order to explore the mechanism of HMOX1 on ischemic stroke, we used OGD/R to induce activation of mouse microglia (BV-2 cells) to simulate ischemic stroke in vitro, followed by the exploration of BV-2 cell biological function changes and detection of the damage of mouse hippocampal neurons HT22 cells. This evidence concerns the gene HMOX1 and ischemic stroke.