Supplementary Figure 1C exhibits the upregulation of HK2, but not endogenous HK2, protein after infection with Ad-HK2FL and Ad-HK2ΔN. On the other hand, confocal immunofluorescent studies (Supplementary Figure 1D) indicate that there is a significant binding of HK2 to the mitochondria when incubated with Ad-HK2FL adenovirus rather than Ad-HK2ΔN adenovirus. Figures 2A-D demonstrate the potential role of HK2FL in aggressive behavior of RA FLS by looking at invasion (Figures 2A, B) and migration (Figures 2C, D). This evidence concerns the gene HK2 and rheumatoid arthritis.