TRIB1 and glioblastoma: We performed double thymidine block in U87 MG GBM cells as shown in Fig. 6a and observed that TRIB1 levels peaked at 8 h after release (1.5 ± 0.1) at which point the cells were shown to be at G2/M phase in the cell cycle as revealed by flow cytometry (Supplementary Fig. 6c).