Moreover, B2M overexpression was also elevated in vitro after αKG treatment (Figure 2F) and positively correlated with GDH1 expression in RCC clinical samples (Figure 2G) and KIRC database (Figure S1H, Supporting Information), accompanied by accumulation of 5hmc in the nucleus (Figure 2H) and GDH1 in the cytoplasm (Figure 2I), the molecules that are positively correlated with intracellular αKG contents.[14] Taken together, these results strongly suggest that αKG can promote B2M expression in RCC Recal cells. This evidence concerns the gene GLUD1 and renal cell carcinoma.