After adjusting for covariates, including age, BMI, years of infertility, gravity, LH and FSH levels, Gn dose, number of oocytes, and 2PN fertilisation rate, using binary logistic regression analysis, the r-ICSI freeze-all ET method showed a higher likelihood of better obstetric outcomes, compared with those of the fresh ET method for LBR [OR:11.77, 95% CI (8.42–16.45)], cLBR [OR:11.29, 95% CI (7.84–16.27)] and conservative cLBR [OR:2.55, 95% CI (1.83–3.55)] (Table 2). The gene discussed is PLOD1; the disease is Infertility.