In all, our investigation about oncogenic role of SOX2-OT via down-regulation of miR-122-3p/FOXO1 and miR-194-5p/FOXA1 axes supports the hypothesis that SOX2-OT as an oncogene might be a novel potential target for NSCLC early detection and treatment. This evidence concerns the gene FOXO1 and non-small cell lung carcinoma.