NOS2 and diabetes mellitus: Moreover, the mRNA expression levels of Wnt/β-catenin target genes and angiogenesis-related genes, including Axin2, Fosl1, Tcf7l2, Vegfa, Flk1, hypoxia-inducible factor 1 alpha (Hif1a), and Tfgb1, were reduced, whereas inducible nitric oxide synthase (Nos2) and inflammatory marker matrix metallopeptidase 9 (Mmp9) were elevated in the wound tissues of HFD+STZ-induced diabetic mice (Fig. 2f).