To study hepatic Thrap3 function in vivo, especially in the NAFLD mouse model, we generated liver-specific Thrap3 knockout mice (referred to as ‘Thrap3 LKO’) by crossing floxed Thrap3 mice (referred to as ‘wild’ or ‘Thrap3 F/F’) with albumin-Cre mice21 (Supplementary Fig. 2a). The gene discussed is THRAP3; the disease is metabolic dysfunction-associated steatotic liver disease.