Considering expression of OCN in highly differentiated cells60,63, the greater expression of OCN on Day21 (162.0 and 478.7% for C.SC and KC.SC, respectively, compared with B.SC sample) compared with Day14 (90.0 and 180.0% for C.SC and KC.SC, respectively, compared with B.SC sample) and ALP activity suggested that BMSCs started mineralization and calcification phase63,64. The gene discussed is BGLAP; the disease is keratoconus.