We performed anti-NLRP3 and anti-F4/80 immunofluorescence staining on esophageal tissue sections to identify the source of NLRP3-regulated IL-18 in EoE and found induced expression of anti-NLRP3 (green) in epithelial cells and anti-NLRP3+anti-F4/80 (red) in macrophages in the A. fumigatus-challenged mice (Fig. 1a, i–ii). This evidence concerns the gene NLRP3 and eosinophilic esophagitis.