To confirm that the biopsies analyzed were indeed from active EoE patients, we performed anti-EPX immunostaining analysis and found high counts of esophageal intact eosinophils (yellow arrow) and extracellular eosinophilic granules (blue arrows) in active EoE patient biopsies compare to no detectable eosinophils in normal individual in the tissue sections (Fig. 6e, i–ii). This evidence concerns the gene EPX and eosinophilic esophagitis.