DEF6 and pulmonary vascular congestion: However, DEF6 overexpression significantly deteriorated pressure overload-induced cardiac hypertrophy and pulmonary congestion, as shown by increments in HW, HW/BW, LW/BW, HW/TL (Fig. 3B), gross heart sizes, and cardiomyocyte cross-sectional area versus the control (AAV9-vector) group (Fig. 3C).