To explore the overall auxo-action of DEF6 in pathological cardiac hypertrophy, cardiomyocyte-specific DEF6-overexpressing mice (AAV9-DEF6) were constructed via tail vein injection of an AAV9 vector containing the DEF6 gene controlled by the cardiomyocyte-specific promoter cTNT. This evidence concerns the gene DEF6 and cardiac hypertrophy.