NR3C1 and plasma cell myeloma: To investigate the genomic features associated with GR binding in malignant plasma cells, we conducted chromatin immunoprecipitation sequencing (ChIP-seq) for GR, and acetylation of histone H3 at lysine 27 (H3K27ac), assay for transposable-accessible chromatin sequencing (ATAC-seq), and RNA sequencing (RNA-seq) in the Dex-sensitive human myeloma cell line MM.1S exposed to Dex (0.1 μM) or to equal-volume ethanol (EtOH) for 1 and 4 h (Fig 1A).