Although we did not detect any difference in Iba1-positive microglia and the number of cells expressing α5 integrin in the spinal cord of mice treated with α5 integrin antibody compared to the isotype control, the decrease in Iba1 immunoreactivity suggests that anti-α5 integrin treatment may have played a role in mitigating neuroinflammation in ALS mice. Here, AIF1 is linked to amyotrophic lateral sclerosis.