The mRNA and protein levels of ZO-1 and Occludin in the M group decreased compared to the NC group (P<0.05), which increased after low-dose TB treatment (P<0.05), and the protein level of Occludin in TL group was significantly higher than that in TH group (P<0.05), (Fig 8E–8I). The gene discussed is TJP1; the disease is tuberculosis.