The results of the analysis of MC3R gene mRNA expression based on the cq value using the Livak Eq. (2–∆∆Cq) showed that the gene expression value of the active TB group increased 3 times compared to the healthy TB group and the contact group and was significantly different with the p value = 0.007 (see Table 2 and Fig. 2). Here, MC3R is linked to tuberculosis.