This study demonstrated that mangiferin (1) alleviated 3-NP induced body weight changes (increased body weight gain), behavioural deficits (improved anxiety-like behaviour, recognition memory, locomotor activity, neurological scoring, rotarod performance and grip strength), (2) restored 3-NP induced biochemical alteration (decreased MDA level, increased level of GSH, enhanced SDH, SOD and CAT activities), and (3) mitigated 3-NP induced histopathological changes (mild neuronal degeneration) in rats. This evidence concerns the gene SOD1 and Anxiety.