To investigate more comprehensively how Menin inhibition affects differentiation and the distribution of subpopulations of leukemia cells in NUP98::NSD1 (FLT3-ITD+) and NUP98::TOP1 (WT1+, CEBPA+) primary AML cells, we examined the surface expression of CD14, CD33, CD11b, CD38, CD90, CD34, CD117, and CD45RA. This evidence concerns the gene CD33 and leukemia.