To explore this in the context of NF1, we stably transfected NF1NS/− Schwann cells and human immortalized Schwann cells from an NF1 nonsense mutation-associated plexiform neurofibroma (iPNF95.6, ATCC CRL-3389) with a dual luciferase reporter (DLR) construct containing five codons of NF1 sequence surrounding the NF1R1947∗ mutation.27 Here, NF1 is linked to plexiform neurofibroma.