The infection with Mtb increases CD4+TNF-α+, CD4+IFN-γ+, and CD4+IL-17+ in the lymph nodes of rBCG-LTAK63 immunized animals (Figure 5C), while in the lungs, there was a drastic difference in CD4+IFN-γ+, and CD4+IL-17+, when compared with BCG (Figure 5D). This evidence concerns the gene TNF and infection.