At 6 h PI, animals with HMGB1 levels ≥24.10 ng/ml were associated with a 13-fold higher risk of ARDS [OR:13.57 (2.76–104.3), p=0.01] and animals with HMGB1 levels ≥31.39 ng/ml were associated with a 12-fold higher risk of mortality [OR: 12.00 (2.36–93.47), p = 0.01] (Table 3). This evidence concerns the gene HMGB1 and acute respiratory distress syndrome.