Of all other virulence genes with a higher prevalence in severe compared to less severe cases in our collection, a statistically significant higher prevalence in HUS cases was only identified for espI and cif. Insufficient data (i.e., expected values were lower than 5) were available to investigate statistical significance for the higher prevalence of iha, ehxA, cba, subA, cma, capU, aggA, aggB, aggC, aggD, aggR, iroN, pic, sepA, aaiC, aap, aar, and aatA in severe disease. This evidence concerns the gene ESPL1 and hemolytic-uremic syndrome.