Of the virulence genes detected across the sub-collection (n: 47), 76.6% or 36 genes were more prevalent in HUS cases than in isolates that caused less severe symptoms, i.e., stx2, eae, iha, ehxA, efa1, astA, espA, espB, espF, espI, espJ, espP, nleA, nleB, nleC, lpfA, katP, tccP, cif, tir, toxB, aggA, aggB, aggC, aggD, aggR, pic, sepA, aaiC, aap, aar, aatA, capU, cba, cma, and iroN (Supplementary Table S11 with gene names in green). Here, ESPL1 is linked to hemolytic-uremic syndrome.