The mRNA levels of ACTG1, CALM3, COL1A2, RPL27A, HNRNPA2B1, CLU, PTMA, PPP2CA, C1S, SERPING1 and LTBP2 were higher in IDD groups than in healthy control in both NP tissues and AF tissues. The gene discussed is C1S; the disease is intervertebral disk degenerative disorder.