The qPCR analysis of glycolysis and glucose transport genes showed that the expression levels of hepatic glucokinase (gck), pyruvate kinase L/R (pklr), and glucose transporter 2 (glut2) were all significantly increased in HCD group at 4th, 6th, and 8th week, while 6-phosphofructokinase (6pfk) only significantly increased at 2nd week, compared to those in NCD group (P < 0.05) (Figure 7(a)–7(d)). This evidence concerns the gene GCK and heavy chain disease.