The immunostaining of hepatic ChREBP showed that the percentage of ChREBP positive hepatocytes and the fluorescence intensity of ChREBP were all significantly increased in HCD group at 2nd, 4th, 6th, and 8th week, compared to those in NCD group (P < 0.05) (Figure 6(a)–6(c)). Here, MLXIPL is linked to heavy chain disease.