HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD (<i>P</i> < 0.05).<h4>Conclusions</h4>HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD, which may be achieved by up-regulating p53 and survivin protein expression and down-regulating pRb protein expression. The gene discussed is BIRC5; the disease is metastatic malignant neoplasm in the lymph nodes.