sPD-L1 was measured using ELISA before and after neoadjuvant chemoradiotherapy in 117 patients with rectal cancer in a study from the UK (61), which indicated that after neoadjuvant chemoradiotherapy, sPD-L1 levels significantly increased and high sPD-L1 levels before neoadjuvant chemoradiotherapy were related to younger age. The gene discussed is SPDL1; the disease is rectal cancer.