In order to better understand the function of HMGB1 in the drug resistance of NSCLC, we established the mouse xenograft model with subcutaneously injecting A549, A549-pcDNA3.1-NC, A549-pcDNA3.1-HMGB1, A549-DDP, A549-DDP-siRNA-NC, and A549-DDP-si-HMGB1 cells. The gene discussed is HMGB1; the disease is non-small cell lung carcinoma.