For example, the R116C mutant of human HspB4 is linked to cataract disease, resulting in nonnegligible changes in the structures, chaperone activity, and oligomerization trend (23), while the R116K mutant retains similar properties to HspB4 WT, indicating that the basic amino acid in that position is essential for maintaining HspB4 activities (24). The gene discussed is CRYAA; the disease is cataract.