After the prompted in-depth analysis of immune cells between the tumor microenvironment and tumor-draining lymph nodes, we found that the number of PD-1+ CD8+ T cells and LAG-3+ CD8+ T increased, leading to the immunosuppressive microenvironment to diminish the onset of the anti-tumor effect after the further booster injections of APE/OVA. Here, PDCD1 is linked to neoplasm.