Based on the above results, we hypothesize that the low expression of KAT2A in ALIS I melanoma cell lines inhibits pathways related to JQ-1 resistance, while high HAT1 expression was unable to regulate the immune microenvironment because there were no immune cells in the culture bottles, which led to the ALIS I cell line’s sensitivity to JQ-1. The gene discussed is KAT2A; the disease is melanoma.