The chloroform fraction also inhibited the growth of A549 spheroids by suppressing the spheroid size, inducing apoptosis, reducing the proportion of CD44 lung cancer stem cells, causing arrest at the S phase of the cell cycle, and suppressing the expression of the pluripotency genes (SOX2 and MYC) that control the stemness of lung cancer stem cells. The gene discussed is MYC; the disease is lung cancer.