SPAM1 and infection: After 48 h of infection with the recombinant viruses rPRV-gG−-TK−-gEgI−, rPRV-IL-18-γ, rPRV-PH20 and rPRV-IL-18-γ-PH20 with an MOI = 10, the mean cell survival rates of the Pan02 cell lines were 81.04%, 69.68%, 25.26% and 5.53% (Figure 4A); the mean cell viability of the EMT-6 cell line was 80.34%, 71.31%, 53.89% and 19.31% (Figure 4B); the mean cell viability of the CT-26 cell line was 82.53%, 76.31%, 65.37% and 30.37% (Figure 4C) and the mean cell survival rates for the H446 cell line were 55.87%, 62.45%, 40.25% and 17.50%, respectively (Figure 4D).