Among them, the tumor volume of mice treated with recombinant virus rPRV-gG−-TK−-gEgI− was reduced by one-quarter compared to the control group, and the tumor growth rate of mice treated with rPRV-IL-18-γ and rPRV-PH20 was further inhibited but did not reach a significant difference compared to rPRV-gG−-TK−-gEgI−. The gene discussed is SPAM1; the disease is neoplasm.