SPAM1 and neoplasm: In the present study, the results of HE staining and IHC staining obviously showed that rPRV-PH20 and rPRV-IL-18-γ-PH20, which carried the PH20 gene, effectively induced widespread tumor cell necrosis, which was consistent with the study in which PH20 is armed in oncolytic adenoviruses for treating glioblastoma and pancreatic cancer [12,23].